Difference between revisions of "Part:BBa K3714010"

Revision as of 02:21, 22 October 2021

Improved gard-337 for lateral flow assay

To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor. This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Figure.1 | Verification of temporarily inhibition and disinhibition by oligonucleotides. The aptazyme was produced by a T7 in vitro transcription system. The product was run on a denatured PAGE. Gardiquimod is an inhibitor of the cleavage activity. "stop" and "start" are two oligonucleotides designed to control the activity of the aptazyme/

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 <partinfo>BBa_K3714010 short</partinfo>
-To test our optimization design with TMSD, a structureless toehold site between biotin probe binding site and aptazyme domain was need. However, designing the probe with manual work and test with available RNA structure prediction model is such a huge work with plenty of attempts.
+To test our optimized design with TMSD(toehold mediated strand displacement) reaction, a toehold site between biotin probe binding site and aptazyme domain is necessary. However, designing the probe manually and testing with available RNA structure prediction model is a huge work with consuming much labor.
-This part is designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed probe shows no influence on the function of aptazyme, the toehold worked as our expect as well.
+This part was designed by our probe design model and verified by the prediction model of team: SJTU-Software. And the changed sequences shows no influence on the function of aptazyme, the toehold worked as our expect as well (Figure.1).
 <!-- Add more about the biology of this part here