Difference between revisions of "Part:BBa K4092007"
Shani leyva (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
| − | <partinfo> | + | <partinfo>BBa_K4092007 short</partinfo> |
Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -28.89999962 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team. | Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -28.89999962 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team. | ||
| Line 18: | Line 18: | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K4092007 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K4092007 parameters</partinfo> |
<!-- --> | <!-- --> | ||
Revision as of 02:04, 22 October 2021
Toehold for Fusarium oxysporum-2
Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Fusarium oxysporum (28S-18S ribosomal RNA intergenic spacer), has a MFE of -28.89999962 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
Usage and Biology
In order to have a more graphical approach of the comparison of the structures, we obtained an image of the secondary structure of the toeholds using the NUPACK package.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]