Difference between revisions of "Part:BBa K3861013"

 
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<partinfo>BBa_K3861013 short</partinfo>
 
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Signal sequence of the AIDA-I autotransporter derived from pRAIDA2.<sup>1</sup> Will be cleaved prior to secretion of the autotransporter.
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Must be used together with the autotransporter to allow display of a protein of interest (POI) on the bacterial surface. The gene encoding for the POI is to be inserted downstream of the signal sequence and upstream of the autotransporter. The AIDA-I autotransporter part can be found here: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861016">BBa_K3861016</a>.
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We combined the parts to create a construct with the potential ability to display the recombinant lectin rBC2LCN (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861001">BBa_K3861001</a>) on the surface of bacterial minicells in order to enable active targeting of MCF7 breast cancer cells by attaching to glycans on the cancer cell surface (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861018">BBa_K3861018</a>).
  
 
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<partinfo>BBa_K3861013 parameters</partinfo>
 
<partinfo>BBa_K3861013 parameters</partinfo>
 
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==='''References'''===
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1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, <I>ACS Synthetic Biology</I>, (2021).
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Revision as of 01:26, 22 October 2021


AIDA signal sequence

Signal sequence of the AIDA-I autotransporter derived from pRAIDA2.1 Will be cleaved prior to secretion of the autotransporter. Must be used together with the autotransporter to allow display of a protein of interest (POI) on the bacterial surface. The gene encoding for the POI is to be inserted downstream of the signal sequence and upstream of the autotransporter. The AIDA-I autotransporter part can be found here: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861016">BBa_K3861016</a>. We combined the parts to create a construct with the potential ability to display the recombinant lectin rBC2LCN (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861001">BBa_K3861001</a>) on the surface of bacterial minicells in order to enable active targeting of MCF7 breast cancer cells by attaching to glycans on the cancer cell surface (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861018">BBa_K3861018</a>).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 32


References

1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, ACS Synthetic Biology, (2021).