Difference between revisions of "Part:BBa K4061200"
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===Design Notes=== | ===Design Notes=== | ||
The design considerations for each part involved could be seen at their respective pages. This device compiles these parts. We used constitutive promoter to drive the expression of both engineered proteins (proteins with modified domains) while kept the scaffold under an inducible promoter. Teams however needed to visit each part (protein domain) and attach the domain themselves next to their protein CDS while designing their parts. | The design considerations for each part involved could be seen at their respective pages. This device compiles these parts. We used constitutive promoter to drive the expression of both engineered proteins (proteins with modified domains) while kept the scaffold under an inducible promoter. Teams however needed to visit each part (protein domain) and attach the domain themselves next to their protein CDS while designing their parts. | ||
| − | + | - The basic parts under this device have been characterised by Whitaker, W. R. et al, however this configuration is novel and relevant wet lab work is in progress | |
Latest revision as of 01:01, 22 October 2021
Scaffold gene kit
This is a device prepared for future teams that seek to enhance protein-protein interactions. In this device, we have compiled parts for the modular domains- SH3 binding domain (BBa_K4061110) and the Leucine Zipper domain (BBa_K4061115). Teams must use these modular protein sequences along with their protein CDS (without stop codon) and express these two interacting engineered proteins under a promoter of interest. Here, they are shown to be constitutively expressed. A scaffold protein is required for fortifying the interaction. This scaffold (BBa_K4061015) could be expressed under any desired promoter. In this kit, it is regulated by an inducible promoter- induced by IPTG addition.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 214
Illegal SapI.rc site found at 374
Design Notes
The design considerations for each part involved could be seen at their respective pages. This device compiles these parts. We used constitutive promoter to drive the expression of both engineered proteins (proteins with modified domains) while kept the scaffold under an inducible promoter. Teams however needed to visit each part (protein domain) and attach the domain themselves next to their protein CDS while designing their parts. - The basic parts under this device have been characterised by Whitaker, W. R. et al, however this configuration is novel and relevant wet lab work is in progress
Source
Whitaker, W. R. et al. "Engineering Robust Control Of Two-Component System Phosphotransfer Using Modular Scaffolds". Proceedings Of The National Academy Of Sciences, vol 109, no. 44, 2012, pp. 18090-18095. Proceedings Of The National Academy Of Sciences, https://doi.org/10.1073/pnas.1209230109.