Difference between revisions of "Part:BBa J119449"
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<h4>Charcterization and improvement in 2021 by SJTU-BioX-Shanghai</h4><br/>
 
<h4>Charcterization and improvement in 2021 by SJTU-BioX-Shanghai</h4><br/>
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In 2021, SJTU-BioX-Shanghai improved the sensibility of this part, see <html><a href="https://parts.igem.org/Part:BBa_K3714004">BBa_K3714004</a></html> for more details.
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In 2021, SJTU-BioX-Shanghai improved the sensibility of this part, see <html><a href="https://parts.igem.org/Part:BBa_K3714004">BBa_K3714004</a></html> for more details. In short, we improved the performance  (cleave fraction) with theophylline present <i>in vitro</i> by introducing some mutation in the loops of aptazyme.  
  
  

Revision as of 00:53, 22 October 2021


N Clone of Mammal Ribozyme

The N Clone of Mammal Ribozyme is a riboswitch that cleaves itself in the presence of theophylline. It has a five nucleotide (nt) base change from the Mammal Ribozyme (J119446). At nt 77-81, the original ribozyme sequence of CATAA was edited to AATAA. It is cloned into the pSB1A2-BR vector, allowing for gene expression using a T7 promoter. The sequence for the original Mammal Ribozyme is found in Massively Parallel RNA Device Engineering in Mammalian Cells with RNA-Seq (https://www.nature.com/articles/s41467-019-12334-y).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Charcterization and improvement in 2021 by SJTU-BioX-Shanghai


In 2021, SJTU-BioX-Shanghai improved the sensibility of this part, see BBa_K3714004 for more details. In short, we improved the performance (cleave fraction) with theophylline present in vitro by introducing some mutation in the loops of aptazyme.