Difference between revisions of "Part:BBa K3945014"
(→‎Usage and Biology)
Line 5: Line 5:
 
__TOC__
 
__TOC__
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
<p>Xpress is an easy-to-use expression vector for <I>E. coli</i> BL21 system designed by the Schryvers’s lab. With a T7 promoter, this vector is easily inducible with IPTG or lactose. Two unique features set this expression vector apart from the other E. coli vectors: a quick screening system allows for fast cloning of genetic constructs into <I>E. coli</i> and a Glutathione S-transferase tag (GST) for easy expression of difficult proteins.</p>
  
 
===Design===
 
===Design===

Revision as of 00:50, 22 October 2021

Xpress: T7-Compatible Expression Vector for E. coli with A GST solubility Tag


Usage and Biology

Xpress is an easy-to-use expression vector for E. coli BL21 system designed by the Schryvers’s lab. With a T7 promoter, this vector is easily inducible with IPTG or lactose. Two unique features set this expression vector apart from the other E. coli vectors: a quick screening system allows for fast cloning of genetic constructs into E. coli and a Glutathione S-transferase tag (GST) for easy expression of difficult proteins.

Design

Experimental Workflow

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
    Illegal NotI site found at 3
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal BamHI site found at 757
    Illegal XhoI site found at 912
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 33
    Illegal XbaI site found at 1908
    Illegal PstI site found at 2955
    Illegal PstI site found at 3842
    Illegal NgoMIV site found at 2201
    Illegal NgoMIV site found at 2598
    Illegal NgoMIV site found at 2608
    Illegal NgoMIV site found at 2734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3694
    Illegal SapI.rc site found at 160
    Illegal SapI.rc site found at 2705

References