Difference between revisions of "Part:BBa K4088898"
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Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.<br/> | Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.<br/> | ||
− | For more information, see <partinfo>BBa_K4088891</partinfo>. | + | For more information, see our part <partinfo>BBa_K4088891</partinfo>. |
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Revision as of 00:49, 22 October 2021
dCas13a
Cas13a is a classic RNA-targeting nuclease that is widely used in a variety of diagnostics methods. It is a very specific variant of RNA detection, it can be customized to recognize single nucleotides on the target RNA. dCas13a is a catalytically dead LwaCas13a enzyme.
For more information, see our part BBa_K4088891.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 622
Illegal BglII site found at 924
Illegal BglII site found at 1689
Illegal BglII site found at 1956
Illegal BglII site found at 2166 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 622
Illegal PstI site found at 601 - 1000COMPATIBLE WITH RFC[1000]