Difference between revisions of "Part:BBa K3795000:Experience"
Isisbnsilva (Talk | contribs) (→Applications of BBa_K3795000) |
Isisbnsilva (Talk | contribs) (→Applications of BBa_K3795000) |
||
| Line 7: | Line 7: | ||
Considering the wet lab, we performed two Immunoassays: one anti-dengue and one anti-Zika western blot with DME-C. The anti-dengue was to confirm that our protein was really capable of detecting dengue virus, and the result was positive so we are really capable of detecting dengue. But to verify if cross reaction was occurring, we performed a anti-zika western blot, and a band appeared, although it can possibly indicate cross reaction, considering that the experiment was performed on a membrane used first to other western blot assay, there may have been an error related to washing, at the time the stripping was performed, the antibodies were not completely removed, and therefore when Zika's membrane was revealed, the result was not the expected. So, for a better precision, the experiment must be done again, with a new membrane, avoiding interference. | Considering the wet lab, we performed two Immunoassays: one anti-dengue and one anti-Zika western blot with DME-C. The anti-dengue was to confirm that our protein was really capable of detecting dengue virus, and the result was positive so we are really capable of detecting dengue. But to verify if cross reaction was occurring, we performed a anti-zika western blot, and a band appeared, although it can possibly indicate cross reaction, considering that the experiment was performed on a membrane used first to other western blot assay, there may have been an error related to washing, at the time the stripping was performed, the antibodies were not completely removed, and therefore when Zika's membrane was revealed, the result was not the expected. So, for a better precision, the experiment must be done again, with a new membrane, avoiding interference. | ||
| − | + | "https://2021.igem.org/wiki/images/e/ea/T--Rio_UFRJ_Brazil--fig8labwork.png" style="width:100%" | |
| − | + | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:46, 22 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3795000
Considering the wet lab, we performed two Immunoassays: one anti-dengue and one anti-Zika western blot with DME-C. The anti-dengue was to confirm that our protein was really capable of detecting dengue virus, and the result was positive so we are really capable of detecting dengue. But to verify if cross reaction was occurring, we performed a anti-zika western blot, and a band appeared, although it can possibly indicate cross reaction, considering that the experiment was performed on a membrane used first to other western blot assay, there may have been an error related to washing, at the time the stripping was performed, the antibodies were not completely removed, and therefore when Zika's membrane was revealed, the result was not the expected. So, for a better precision, the experiment must be done again, with a new membrane, avoiding interference.
"
" style="width:100%"
User Reviews
UNIQc0659f072fd4cf34-partinfo-00000000-QINU UNIQc0659f072fd4cf34-partinfo-00000001-QINU