Difference between revisions of "Part:BBa K3814075"

Latest revision as of 00:39, 22 October 2021

Cluster 7

We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.

To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.

We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 716
Illegal NheI site found at 739
Illegal NheI site found at 1970
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2805
Illegal XhoI site found at 75
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 420
Illegal AgeI site found at 1106
Illegal AgeI site found at 2271
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3814075 short</partinfo>
-Cluster 7
+We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.
+To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.
+We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.
 <!-- Add more about the biology of this part here