Difference between revisions of "Part:BBa K3814071"

Latest revision as of 00:36, 22 October 2021

Cluster 3

We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.

To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.

We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 4216
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 4268
Illegal NheI site found at 4291
Illegal SpeI site found at 4216
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4731
Illegal XhoI site found at 75
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 4216
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 4216
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 691

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3814071 short</partinfo>
-Cluster 3
+We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.
+To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.
+We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.
 <!-- Add more about the biology of this part here