Difference between revisions of "Part:BBa K3805329"
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<partinfo>BBa_K3805329 short</partinfo> | <partinfo>BBa_K3805329 short</partinfo> | ||
− | We combined three kinds of proinsulin | + | <html> |
+ | We combined three kinds of proinsulin Normal<a href = "https://parts.igem.org/Part:BBa_K3805429"> (BBa_K3805429)</a>, Mutation Ⅰ<a href = "https://parts.igem.org/Part:BBa_K3805666"> (BBa_K3805666)</a>, Mutation Ⅱ<a href = "https://parts.igem.org/Part:BBa_K3805001"> (BBa_K3805001)</a> with araC <a href = "https://parts.igem.org/Part:BBa_I0500">(BBa_I0500)</a>, letting proinsulin sequence affect the expression of araC. Here we take proinsulin(Normal) as an example. | ||
+ | </html> | ||
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Latest revision as of 00:16, 22 October 2021
Fusion protein with proinsulin and araC
We combined three kinds of proinsulin Normal (BBa_K3805429), Mutation Ⅰ (BBa_K3805666), Mutation Ⅱ (BBa_K3805001) with araC (BBa_I0500), letting proinsulin sequence affect the expression of araC. Here we take proinsulin(Normal) as an example.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1487
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1426
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1261
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1243