Difference between revisions of "Part:BBa K175004:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
  
−
The Glutamine codon at 172 was changed from cag to caa in order to remove the PstI cut site.
+
Original gene has a biobrick cut site at 169.
 +
Codon optimized for E.coli with upgene.
  
 
===Source===
 
===Source===

Latest revision as of 11:47, 8 July 2009

I-PpoI homing endonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 166


Design Notes

Original gene has a biobrick cut site at 169. Codon optimized for E.coli with upgene.

Source

From slime mold (Physarum polycephalum), http://www.ncbi.nlm.nih.gov/protein/102295?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

References