Difference between revisions of "Part:BBa K3871001"
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== Description ==
 
== Description ==
 
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     mCherry is a red fluorescent protein used as a reporter derived from the
 
     mCherry is a red fluorescent protein used as a reporter derived from the
 
     fluorophore DsRed, which wasthat originally isolated from
 
     fluorophore DsRed, which wasthat originally isolated from
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     This part is a variant of mCherry, the sequence of which was modified to
 
     This part is a variant of mCherry, the sequence of which was modified to
 
     optimize its expression in <i>Bacillus subtilis</i> against <i>E. coli</i> using the
 
     optimize its expression in <i>Bacillus subtilis</i> against <i>E. coli</i> using the
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     <a href="https://2021.igem.org/Team:TAU_Israel">Communique</a>tool developed
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     <a href="https://2021.igem.org/Team:TAU_Israel" display:"inline;">Communique</a>tool developed
 
     by the 2021 TAU Israel team. This specific variant was optimized based on
 
     by the 2021 TAU Israel team. This specific variant was optimized based on
 
     codon usage bias in highly expressed genes in <i>B. subtilis</i>. You may
 
     codon usage bias in highly expressed genes in <i>B. subtilis</i>. You may
 
     read more about the optimization model
 
     read more about the optimization model
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     <a href="https://2021.igem.org/Team:TAU_Israel/Model">here.</a>
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     <a href="https://2021.igem.org/Team:TAU_Israel/Model" display:"inline;">here.</a>
 
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===Biology===
 
===Biology===
 
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   <p>Results of the optimization are described in the figures below. For more information on the results, please consult the 2021 TAU Israel wiki results page found <a href = "https://2021.igem.org/Team:TAU_Israel/Results">here.</a>Information about experimental procedures can be found <a href = "https://2021.igem.org/Team:TAU_Israel/Notebook">here</a>and <a href = "https://2021.igem.org/Team:TAU_Israel/Proof_Of_Concept">here.</a></p>
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   <p>Results of the optimization are described in the figures below. For more information on the results, please consult the 2021 TAU Israel wiki results page found <a href = "https://2021.igem.org/Team:TAU_Israel/Results" display:"inline;">here.</a>Information about experimental procedures can be found <a href = "https://2021.igem.org/Team:TAU_Israel/Notebook" display:"inline;">here</a>and <a href = "https://2021.igem.org/Team:TAU_Israel/Proof_Of_Concept" display:"inline;">here.</a></p>
 
   
 
   
 
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Revision as of 00:07, 22 October 2021

Description

mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which wasthat originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.

This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communiquetool developed by the 2021 TAU Israel team. This specific variant was optimized based on codon usage bias in highly expressed genes in B. subtilis. You may read more about the optimization model here.


mCherry, CAI optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Biology

Results of the optimization are described in the figures below. For more information on the results, please consult the 2021 TAU Israel wiki results page found here.Information about experimental procedures can be found hereand here.