Difference between revisions of "Part:BBa K3871001"
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== Description ==
 
== Description ==
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<html><p align=justify>mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which wasthat originally isolated from <i>Discosoma</i> sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.</p>
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<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum</p></html>
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  <p align="justify">
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    mCherry is a red fluorescent protein used as a reporter derived from the
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    fluorophore DsRed, which wasthat originally isolated from
 +
    <i>Discosoma</i> sea anemones. It is commonly used due to its colour and
 +
    photostability compared to other monomeric fluorophores.
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  </p>
  
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  <p align="justify">
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    This part is a variant of mCherry, the sequence of which was modified to
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    optimize its expression in <i>Bacillus subtilis</i> against <i>E. coli</i> using the
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    <a href="https://2021.igem.org/Team:TAU_Israel">Communique</a>tool developed
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    by the 2021 TAU Israel team. This specific variant was optimized based on
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    codon usage bias in highly expressed genes in <i>B. subtilis</i>. You may
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    read more about the optimization model
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    <a href="https://2021.igem.org/Team:TAU_Israel/Model">here.</a>
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  </p>
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</html>
  
 
__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3871001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3871001 SequenceAndFeatures</partinfo>
  
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===Biology===
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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  <p align="justify">Results of the optimization are described in the figures below. For more information on the results, please consult the 2021 TAU Israel wiki results page found <a href = "https://2021.igem.org/Team:TAU_Israel/Results">here.</a>Information about experimental procedures can be found <a href = "https://2021.igem.org/Team:TAU_Israel/Notebook">here</a>and<a href = "https://2021.igem.org/Team:TAU_Israel/Proof_Of_Concept">here.</a></p>
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<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
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<partinfo>BBa_K3871001 parameters</partinfo>
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Revision as of 23:59, 21 October 2021

Description

mCherry is a red fluorescent protein used as a reporter derived from the fluorophore DsRed, which wasthat originally isolated from Discosoma sea anemones. It is commonly used due to its colour and photostability compared to other monomeric fluorophores.

This part is a variant of mCherry, the sequence of which was modified to optimize its expression in Bacillus subtilis against E. coli using the Communiquetool developed by the 2021 TAU Israel team. This specific variant was optimized based on codon usage bias in highly expressed genes in B. subtilis. You may read more about the optimization model here.


mCherry, CAI optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Biology

Results of the optimization are described in the figures below. For more information on the results, please consult the 2021 TAU Israel wiki results page found here.Information about experimental procedures can be found hereandhere.