Difference between revisions of "Part:BBa K4046610"

Revision as of 23:51, 21 October 2021

hUBC-Kozak-tdTomato

This is a constitutive reporter consisting of the hUBC promoter followed by the gene coding for the tdTomato red fluorescence protein. In between these two sequences is the Kozak sequence, which provides a start point for eukaryotic translation. This construct was added to a pcDNA5 plasmid and was created as one of our constitutive reporters; it will be used as a construct into which we insert our dhdO binding sequences in order to build a dynamic reporter for the compound D-2-HG.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 759
Illegal PstI site found at 1485
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 759
Illegal PstI site found at 1485
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 759
Illegal PstI site found at 1485
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 759
Illegal PstI site found at 1485
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:50, 21 October 2021 (view source) Registry (Talk \| contribs)		Revision as of 23:51, 21 October 2021 (view source) Ayj9 (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4046610 short</partinfo>		<partinfo>BBa_K4046610 short</partinfo>

−	This is a constitutive reporter consisting of the hUBC promoter followed by the gene coding for the tdTomato red fluorescence protein. In between these two sequences is the Kozak sequence, which provides a start point for eukaryotic translation. This construct was added to a pcDNA5 plasmid and was created as one of our constitutive reporters; it will be used as a construct into which we insert our <i>dhdO<\i> binding sequences in order to build a dynamic reporter for the compound D-2-HG.	+	This is a constitutive reporter consisting of the hUBC promoter followed by the gene coding for the tdTomato red fluorescence protein. In between these two sequences is the Kozak sequence, which provides a start point for eukaryotic translation. This construct was added to a pcDNA5 plasmid and was created as one of our constitutive reporters; it will be used as a construct into which we insert our <i>dhdO</i> binding sequences in order to build a dynamic reporter for the compound D-2-HG.