Difference between revisions of "Part:BBa K3783017:Experience"

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   <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>
 
   <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>
 
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<p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
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<p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control
  
 
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Latest revision as of 23:43, 21 October 2021


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Applications of BBa_K3783017

OhioState Application

LAL Assay Graph
Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control

User Reviews

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