Difference between revisions of "Part:BBa K4055517"
 
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<partinfo>BBa_K4055517 short</partinfo>
 
<partinfo>BBa_K4055517 short</partinfo>
  
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This part is used to express Mfp3s-pep protein.  Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of HA.
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This part is used to express Mfp3s-pep protein.  Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of hydroxyapatite (HA).
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[[File:T--Yucai_SZ--Mfp3S-pep.png|center|Mfp3S analog peptide(Mfp3S-pep)]]
  
 
===Experiment===
 
===Experiment===
 
[[File:T--Yucai_SZ--1.png|500px|thumb|center|Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed]]
 
[[File:T--Yucai_SZ--1.png|500px|thumb|center|Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed]]
 
<br><p>
 
<br><p>
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After 9 days of SBF culture, THE MFP-CSGA was scanned by electron microscopy (compared with the sample on day 0).</p><br>
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After 9 days of SBF culture, The mineralized biofilm was scanned by electron microscopy (compared with the sample on day 0).</p><br>
  
 
[[File:T--Yucai_SZ--2.png|500px|thumb|center|SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm]]
 
[[File:T--Yucai_SZ--2.png|500px|thumb|center|SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm]]

Latest revision as of 23:30, 21 October 2021


Mfp3S-pep adhesive protein

This part is used to express Mfp3s-pep protein. Mfp3s-pep （Mussel foot protein peptide）is the protein we selected from the papers of Yi Yu , Yi Cao , Chao Zhong and so on.Mfp3S-pep is enriched with aspartic acid (blue), lysine (green) and tyrosine (red) residues, known for their functional roles in promoting the nucleation, growth and adhesion of hydroxyapatite (HA).

Mfp3S analog peptide(Mfp3S-pep)

Experiment

Staining results of protein expressed by plasmid PT7-B0034-CsgA_linker_Mfp3spep, suggests that there are biofilms that are expressed

After 9 days of SBF culture, The mineralized biofilm was scanned by electron microscopy (compared with the sample on day 0).


SEM image showing the surface morphology of the biofilm (i.e., unmineralized). Scale bars:3μm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




References

Living materials fabricated via gradient mineralization of light-inducible biofilms (DOI:10.1038/s41589-020-00697-z)