Difference between revisions of "Part:BBa K3558000:Experience"
(→‎Growth Assay of BBa_K3558000)
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[[File:T--UNSW Australia--HSP22Efig1.png|500px|thumb|center|Figure 1: Flow diagram of heat shock protein growth assay method. Image created in BioRender.]]
 
[[File:T--UNSW Australia--HSP22Efig1.png|500px|thumb|center|Figure 1: Flow diagram of heat shock protein growth assay method. Image created in BioRender.]]
  
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[[File:T--UNSW Australia--HSP22Efig2new.jpg|500px|thumb|center|Figure 2: Growth curve of E. coli cells per mL at 37ᵒC (A) and 45ᵒC (B) over approximately six hours. Y-axis represents the time measured in minutes, while x-axis represents the number of cells per mL. ]]
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[[File:T--UNSW Australia--HSP22Efig2new.jpg|500px|thumb|center|Figure 2: Growth curve of E. coli cells per mL at 37ᵒC (A) and 45ᵒC (B) over approximately six hours. Y-axis represents the time measured in minutes, while x-axis represents the number of cells per mL.]]
  
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[[File:T--UNSW Australia--HSPfig3.png|500px|thumb|center|Figure 3: Flow diagram of heat shock protein survival assay method. Image created in BioRender.]]
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[[File:T--UNSW Australia--HSP22Efig4.jpg|500px|thumb|center|Figure 4: Survival assay cell concentration at 37℃ and 46.4℃. HSP22E w IPTG stands for E. coli containing HSP22E gene induced to express the gene with IPTG. Similarly, HSP22E w/o IPTG stands for E. coli containing HSP22E gene without IPTG induction. mC3 w IPTG and mC3 w/o IPTG represent the E. coli samples with the gene for mCerulean3 with and without IPTG induction, respectively.]]
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[[File:T--UNSW Australia--HSP22Efig5.png|500px|thumb|center|Figure 5: SDS-PAGE of samples after Bugbuster protein expression. The samples were collected prior to heat shock after 1 hour IPTG induction.]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:04, 21 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3558000

Growth Assay of BBa_K3558000

Figure 1: Flow diagram of heat shock protein growth assay method. Image created in BioRender.
Figure 2: Growth curve of E. coli cells per mL at 37ᵒC (A) and 45ᵒC (B) over approximately six hours. Y-axis represents the time measured in minutes, while x-axis represents the number of cells per mL.
Figure 3: Flow diagram of heat shock protein survival assay method. Image created in BioRender.
Figure 4: Survival assay cell concentration at 37℃ and 46.4℃. HSP22E w IPTG stands for E. coli containing HSP22E gene induced to express the gene with IPTG. Similarly, HSP22E w/o IPTG stands for E. coli containing HSP22E gene without IPTG induction. mC3 w IPTG and mC3 w/o IPTG represent the E. coli samples with the gene for mCerulean3 with and without IPTG induction, respectively.
Figure 5: SDS-PAGE of samples after Bugbuster protein expression. The samples were collected prior to heat shock after 1 hour IPTG induction.

User Reviews

UNIQa4bf3d1801ab5578-partinfo-00000000-QINU UNIQa4bf3d1801ab5578-partinfo-00000001-QINU