Difference between revisions of "Part:BBa K245114:Experience"
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= Functional Test = | = Functional Test = | ||
− |   After finishing the design of DenTeeth, we wanted to know whether its inhibition ability can have a function, so we designed the following experiment. | + |    After finishing the design of DenTeeth, we wanted to know whether its inhibition ability can have a function, so we designed the following experiment. |
+ | |||
<br>  By taking the method of dish culture and sticking up the filter paper, we dropped the DenTeeth on the filter paper in the center of the <i>E. coli</i>, DH5α with pET32a, plate. After twelve hours, a circular area around the spot of the DenTeeth formed, in which the bacteria colonies did not grow. Inhibition zone proved that the LL-37 produced by DenTeeth could successfully secrete out and inhibit the growth of <i>E. coli</i>, DH5α with pET32a. | <br>  By taking the method of dish culture and sticking up the filter paper, we dropped the DenTeeth on the filter paper in the center of the <i>E. coli</i>, DH5α with pET32a, plate. After twelve hours, a circular area around the spot of the DenTeeth formed, in which the bacteria colonies did not grow. Inhibition zone proved that the LL-37 produced by DenTeeth could successfully secrete out and inhibit the growth of <i>E. coli</i>, DH5α with pET32a. | ||
[[File:T--NCTU_Formosa--Zone of inhibition.png|500px|thumb|center|'''Figure 4. DenTeeth inhibition zone result in LB plates. ( The materials added in plate were in the following condition, and each total volume is 20μL. (A) 30% hydrogen peroxide, (B) <i>E. coli</i> (BL21) with pSB1K3, (C) DenTeeth, (D) <i>E. coli</i> (BL21) with pSB1K3 (Cell Lysate), (E) DenTeeth (Cell Lysate), (F) PBS, (G) <i>E. coli</i> (BL21) with pSB1K3 + PBS, (H) DenTeeth + PBS, (I) LB broth (Centrifuging <i>E. coli</i> (BL21) with pSB1K3 after liquid culturing ), (J) LB broth (Centrifuging <i>E. coli</i> (BL21) with pSB1K3 after liquid culturing ) ) | [[File:T--NCTU_Formosa--Zone of inhibition.png|500px|thumb|center|'''Figure 4. DenTeeth inhibition zone result in LB plates. ( The materials added in plate were in the following condition, and each total volume is 20μL. (A) 30% hydrogen peroxide, (B) <i>E. coli</i> (BL21) with pSB1K3, (C) DenTeeth, (D) <i>E. coli</i> (BL21) with pSB1K3 (Cell Lysate), (E) DenTeeth (Cell Lysate), (F) PBS, (G) <i>E. coli</i> (BL21) with pSB1K3 + PBS, (H) DenTeeth + PBS, (I) LB broth (Centrifuging <i>E. coli</i> (BL21) with pSB1K3 after liquid culturing ), (J) LB broth (Centrifuging <i>E. coli</i> (BL21) with pSB1K3 after liquid culturing ) ) | ||
]] | ]] | ||
+ |    Finally, we chose antimicrobial peptides, LL-37 play the part of bacteria-inhibition. Human cathelicidin-derived LL-37 is a 37-residue cationic, amphipathic α-helical peptide. It is an active component of mammalian innate immunity. LL-37 not only can inhibit P. gingivalis but can also inhibit <i>Escherichia coli</i> itself, preventing DenTeeth from overgrowing after entering the canine mouth. | ||
+ | |||
+ | [[File:T--NCTU_Formosa--Zone of inhibition2.png|500px|thumb|center|'''Figure 5. The Bar Chart of DenTeeth inhibition Functional Test. | ||
+ | ]] | ||
|}; | |}; | ||
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Revision as of 22:52, 21 October 2021
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