Difference between revisions of "Part:BBa K4044009"
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<partinfo>BBa_K4044009 short</partinfo>
 
<partinfo>BBa_K4044009 short</partinfo>
  
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there are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1-BcLOV4 becomes active and binds to Gal4-QPAS 1. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, Gal4-QPAS1 remains in the dimerized state. This leads to inhibition of transcription. At the same time, there may be residual activity of BphP1 and expression of target genes, which we do not need, therefore, under the influence of blue light, the entire BphP1-bclov4 complex binds to the lipid membrane, which completely eliminates the possibility of undesirable expression.
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There are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1-BcLOV4 becomes active and binds to QPAS1-Gal4. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, QPAS1-Gal4 remains in the dimerized state. This leads to inhibition of transcription. At the same time, there may be residual activity of BphP1 and expression of target genes, which we do not need, therefore, under the influence of blue light, the entire BphP1-BcLov4 complex binds to the lipid membrane, which completely eliminates the possibility of undesirable expression.
  
 
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Revision as of 22:34, 21 October 2021


BphP1-BcLOV4_QPAS1-Gal4 optogenetic system

There are several states of the ontogenetic system. Under the influence of far red light, BphP1 part of the chimeric protein BphP1-BcLOV4 becomes active and binds to QPAS1-Gal4. This leads to the expression of target gene. Under the influence of red light, BphP1 is inactivated, QPAS1-Gal4 remains in the dimerized state. This leads to inhibition of transcription. At the same time, there may be residual activity of BphP1 and expression of target genes, which we do not need, therefore, under the influence of blue light, the entire BphP1-BcLov4 complex binds to the lipid membrane, which completely eliminates the possibility of undesirable expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 4029
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 1705
  • 1000
    COMPATIBLE WITH RFC[1000]