Difference between revisions of "Part:BBa K3806014:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This composite part constitutes a biosensing unit based on a theophylline aptazyme sequence flanked by a ribosomal binding site and ribosomal binding site complementary sequence (BBa_K3806010). A codon optimized lacZ (BBa_K3806012) acts as a reporter gene. The construct is preceded by a T7 promoter (BBa_K3633015) for transcription by T7 bacteriophage RNA polymerase. | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
Latest revision as of 22:31, 21 October 2021
Theophylline-binding aptazyme regulating lacZ expression (cRBS). With T7 promoter.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3204
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2321
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part constitutes a biosensing unit based on a theophylline aptazyme sequence flanked by a ribosomal binding site and ribosomal binding site complementary sequence (BBa_K3806010). A codon optimized lacZ (BBa_K3806012) acts as a reporter gene. The construct is preceded by a T7 promoter (BBa_K3633015) for transcription by T7 bacteriophage RNA polymerase.