Difference between revisions of "Part:BBa K3783008:Experience"

 
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===Applications of BBa_K3783008===
 
===Applications of BBa_K3783008===
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=OhioState Application=
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  <img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph">
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  <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>
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<p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control
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===User Reviews===
 
===User Reviews===

Latest revision as of 22:30, 21 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3783008

OhioState Application

LAL Assay Graph
Figure 2. Endotoxin Units of Construct Lysates via LAL Assay

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a slight increase in endotoxin activity in the pFraB-LF. However, when looking at the hybrid promoter the endotoxin activity was less than the control

User Reviews

UNIQ7df4be3ae2ae4a4c-partinfo-00000001-QINU UNIQ7df4be3ae2ae4a4c-partinfo-00000002-QINU