Difference between revisions of "Part:BBa K3805639:Design"
 
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===Design Notes===
 
===Design Notes===
 
It is necessary to design DNA sequences with appropriate length to produce mRNA binding switches according to the types of different proteins.
 
It is necessary to design DNA sequences with appropriate length to produce mRNA binding switches according to the types of different proteins.
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Taking human insulin proteinogen as an example, we hope to use this switch of mRNA interaction to ensure the normal growth of the strain without expressing the downstream killing system when the expression level of insulin mRNA is normal, and to start the expression of the downstream killing system and kill the deceiver when the mutation at the key site of proteinogen gene leads to the abnormal expression level of its mRNA. At the same time, we hope to explore the development possibility of mRNA interaction switch, and explore the optimal length ratio and optimal conditions of two mRNA interactions.
+
Taking proinsulin as an example, we hope to use this switch of mRNA interaction to ensure the normal growth of the strain without expressing the downstream killing system when the expression level of insulin mRNA is normal, and to start the expression and kill the cheaters when the mutation at the key site of proinsulin gene leads to the abnormal expression level of its mRNA. At the same time, we hope to explore the development possibility of mRNA interaction switch, and explore the optimal length ratio and optimal conditions of two mRNA interactions.
  
  

Latest revision as of 22:28, 21 October 2021


mRNA binding site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is necessary to design DNA sequences with appropriate length to produce mRNA binding switches according to the types of different proteins. Taking proinsulin as an example, we hope to use this switch of mRNA interaction to ensure the normal growth of the strain without expressing the downstream killing system when the expression level of insulin mRNA is normal, and to start the expression and kill the cheaters when the mutation at the key site of proinsulin gene leads to the abnormal expression level of its mRNA. At the same time, we hope to explore the development possibility of mRNA interaction switch, and explore the optimal length ratio and optimal conditions of two mRNA interactions.


Source

No natural comparator


References