Difference between revisions of "Part:BBa K4044003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool. | + | Codon optimization of the nucleotide sequence for efficient gene expression in ''E. coli'' was performed using GENEWIZ codon optimization tool. |
===Source=== | ===Source=== | ||
− | BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli. | + | BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for ''E. coli''. |
− | Source organism: Botrytis cinerea | + | Source organism: ''Botrytis cinerea'' |
===References=== | ===References=== |
Latest revision as of 22:18, 21 October 2021
BcLOV4
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 354
Illegal PstI site found at 598 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 354
Illegal PstI site found at 598 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 354
Illegal PstI site found at 598 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 354
Illegal PstI site found at 598 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization of the nucleotide sequence for efficient gene expression in E. coli was performed using GENEWIZ codon optimization tool.
Source
BcLOV4 protein sequence was taken from https://www.uniprot.org/uniprot/A0A384J9J8 and codon optimized for E. coli.
Source organism: Botrytis cinerea