Difference between revisions of "Part:BBa K116401"
| Line 9: | Line 9: | ||
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017. | Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017. | ||
| − | [[File:Fjasknfkncjak.png|thumb|center|800px|Figure | + | [[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM. ]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 22:10, 21 October 2021
external phosphate sensing promoter
Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.
Experience
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]