Difference between revisions of "Part:BBa K3739110"

(Usage)
(Usage)
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===Usage===
 
===Usage===
 
By codon optimization, the sequence suitable for expression in ''Vibrio Natriegens'' was constructed. Adding T7-RBS and terminator,construct new composite part <partinfo>BBa_K3739110</partinfo>.We transformed the constructed plasmid into ''Vibrio Natriegens'' to verify its successful heterologous expression.
 
By codon optimization, the sequence suitable for expression in ''Vibrio Natriegens'' was constructed. Adding T7-RBS and terminator,construct new composite part <partinfo>BBa_K3739110</partinfo>.We transformed the constructed plasmid into ''Vibrio Natriegens'' to verify its successful heterologous expression.
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 +
===Characterization===
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 +
====1. Agarose Gel Electrophoresis====
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After transferring the plasmid into ''E.coli'', colony PCR was used to certify the plasmid was correct. The expected result was obtained.
 
[[File:T--XMU-China--K3739110X.png]]
 
[[File:T--XMU-China--K3739110X.png]]
  

Revision as of 22:07, 21 October 2021


K525998(T7-RBS)-LMT-PPO-his-B0010

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.

Biology

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion

Usage

By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739110.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.

Characterization

1. Agarose Gel Electrophoresis

After transferring the plasmid into E.coli, colony PCR was used to certify the plasmid was correct. The expected result was obtained. T--XMU-China--K3739110X.png

Reference

1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.

2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]