Difference between revisions of "Part:BBa K3739110"
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===Usage=== | ===Usage=== | ||
By codon optimization, the sequence suitable for expression in ''Vibrio Natriegens'' was constructed. Adding T7-RBS and terminator,construct new composite part <partinfo>BBa_K3739110</partinfo>.We transformed the constructed plasmid into ''Vibrio Natriegens'' to verify its successful heterologous expression. | By codon optimization, the sequence suitable for expression in ''Vibrio Natriegens'' was constructed. Adding T7-RBS and terminator,construct new composite part <partinfo>BBa_K3739110</partinfo>.We transformed the constructed plasmid into ''Vibrio Natriegens'' to verify its successful heterologous expression. | ||
| + | |||
| + | ===Characterization=== | ||
| + | |||
| + | ====1. Agarose Gel Electrophoresis==== | ||
| + | After transferring the plasmid into ''E.coli'', colony PCR was used to certify the plasmid was correct. The expected result was obtained. | ||
[[File:T--XMU-China--K3739110X.png]] | [[File:T--XMU-China--K3739110X.png]] | ||
Revision as of 22:07, 21 October 2021
K525998(T7-RBS)-LMT-PPO-his-B0010
LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.
Biology
LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion
Usage
By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739110.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.
Characterization
1. Agarose Gel Electrophoresis
After transferring the plasmid into E.coli, colony PCR was used to certify the plasmid was correct. The expected result was obtained.
Reference
1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.
2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]