Difference between revisions of "Part:BBa K3799007"
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<partinfo>BBa_K3799007 short</partinfo> | <partinfo>BBa_K3799007 short</partinfo> | ||
| − | This part consist of the AgrC-AgrA quorum-sensing signal transduction system of S.aureus regulated by pBad promoter from E. Coli(BBa_K206000).The activity of this two-component system in turn regulate P2 promoter which is studied using GFP expression(BBa_K391000) | + | This part consist of the AgrC-AgrA quorum-sensing signal transduction system of S.aureus regulated by pBad promoter from E. Coli([https://parts.igem.org/Part:BBa_K206000 BBa_K206000]).The activity of this two-component system in turn regulate P2 promoter which is studied using GFP expression([https://parts.igem.org/Part:BBa_K391000 BBa_K391000]) |
Latest revision as of 21:56, 21 October 2021
Unidirectional model of AIP-1 sensor of S aureus regulating GFP intensity using P2 promoter
This part consist of the AgrC-AgrA quorum-sensing signal transduction system of S.aureus regulated by pBad promoter from E. Coli(BBa_K206000).The activity of this two-component system in turn regulate P2 promoter which is studied using GFP expression(BBa_K391000)
The regulation of P2 promoter by the two-component system when both Pveg and P2 promoter are aligned in the same direction is studied using this system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 513
Illegal BamHI site found at 65
Illegal BamHI site found at 1397 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2953