Difference between revisions of "Part:BBa K116401"
| Line 6: | Line 6: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| + | |||
====Experience==== | ====Experience==== | ||
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017. | Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017. | ||
| − | + | [[File:Fjasknfkncjak.png|thumb|center|800px|Figure 5: Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM. ]] | |
| − | [[File:Fjasknfkncjak.png|thumb|center|800px|Figure | + | |
Revision as of 21:46, 21 October 2021
external phosphate sensing promoter
Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]