Difference between revisions of "Part:BBa K3783003:Experience"
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<img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph"> | <img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph"> | ||
| − | <figcaption style="text-align:center">Figure | + | <figcaption style="text-align:center">Figure 2. Endotoxin Units of Construct Lysates via LAL Assay</figcaption> |
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Revision as of 21:44, 21 October 2021
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how you used this part and how it worked out.
OhioState Application
The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
===User Reviews===