Difference between revisions of "Part:BBa K116401"

 
Line 6: Line 6:
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
===Usage and Biology===
+
 
 +
====Experience====
 +
Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol under Pho), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.
 +
 
 +
 
 +
[[File:Fjasknfkncjak.png|thumb|center|800px|Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.]]
 +
 
 +
 
 +
 
  
 
<!-- -->
 
<!-- -->

Revision as of 21:42, 21 October 2021


external phosphate sensing promoter

promoter of phoB in E.coli, an external phosphate regulated promoter which can be activated when encountering phosphate limitation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]