Difference between revisions of "Part:BBa K3783003:Experience"

Revision as of 21:41, 21 October 2021

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Applications of BBa_K3783003

OhioState 2021=

 <img src="" class="generalimage" alt="LAL Assay Graph">
 <figcaption style="text-align:center">Figure 1. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>

</figure>

The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.

User Reviews

UNIQ7574c3e59a6796c1-partinfo-00000000-QINU

UNIQ7574c3e59a6796c1-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K3783003===
+==OhioState 2021===
+<figure>
+  <img src="https://2021.igem.org/wiki/images/b/b0/T--OhioState--LALAssayGraph.png" class="generalimage" alt="LAL Assay Graph">
+  <figcaption style="text-align:center">Figure 1. Endotoxin Units of Construct Lysates via LAL Assay</figcaption>
+</figure>
+<p>The 2021 OhioState team characterized this biobrick using a Pierce Chromogenic Endotoxin Quantification Kit from ThermoFisher. The results were treated with Limulus Amebocyte Lysate which binds endotoxin and then attached to a chromogenic substrate which allowed us to quantify our results with provided standards. Cultures diluted to the -5 were used to quantify endotoxin concentrations. A control without any anti-lipid A proteins was also used as a comparison. There was a noticeable decrease in bound endotoxin.
 ===User Reviews===