Difference between revisions of "Part:BBa K3739045"
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===Biology=== | ===Biology=== | ||
The polyphenol oxidase we used is a kind of polyphenol oxidase from the Marine mussel itself, which regulates the mucin production of mussel. It can oxidase the dopamine on the side chain of mussel mucin to dopamine quinone, and its catalytic effect can be characterized by the change of viscosity characteristics of mussel mucin. | The polyphenol oxidase we used is a kind of polyphenol oxidase from the Marine mussel itself, which regulates the mucin production of mussel. It can oxidase the dopamine on the side chain of mussel mucin to dopamine quinone, and its catalytic effect can be characterized by the change of viscosity characteristics of mussel mucin. | ||
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| + | [[File:T--XMU-China--PPOF1.png|400px]] | ||
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| + | :'''Fig.1.''' the mechanism diagram of PPO | ||
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===Usage=== | ===Usage=== | ||
According to the biological characteristics of VnDX, we optimized the codon and added a 6His-tag to construct a sequence suitable for expression in VnDX. We hope that it can successfully express polyphenol oxidase in VnDX and reduce the viscosity characteristics of mussel mucin. | According to the biological characteristics of VnDX, we optimized the codon and added a 6His-tag to construct a sequence suitable for expression in VnDX. We hope that it can successfully express polyphenol oxidase in VnDX and reduce the viscosity characteristics of mussel mucin. | ||
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| + | ===Reference=== | ||
| + | 1. Sendovski, M.; Kanteev, M.; Ben-Yosef, V. S.; Adir, N.; Fishman, A., First structures of an active bacterial tyrosinase reveal copper plasticity. J Mol Biol 2011, 405 (1), 227-37. | ||
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Revision as of 21:32, 21 October 2021
J23100-B0030-PPO-his-B0010
The polyphenol oxidase efficiently catalyzes the reaction of oxidizing dopamine to dopa quinone. His-tag was added to purify the protein.
Biology
The polyphenol oxidase we used is a kind of polyphenol oxidase from the Marine mussel itself, which regulates the mucin production of mussel. It can oxidase the dopamine on the side chain of mussel mucin to dopamine quinone, and its catalytic effect can be characterized by the change of viscosity characteristics of mussel mucin.
- Fig.1. the mechanism diagram of PPO
Usage
According to the biological characteristics of VnDX, we optimized the codon and added a 6His-tag to construct a sequence suitable for expression in VnDX. We hope that it can successfully express polyphenol oxidase in VnDX and reduce the viscosity characteristics of mussel mucin.
Reference
1. Sendovski, M.; Kanteev, M.; Ben-Yosef, V. S.; Adir, N.; Fishman, A., First structures of an active bacterial tyrosinase reveal copper plasticity. J Mol Biol 2011, 405 (1), 227-37.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]