Difference between revisions of "Part:BBa K3739063"

Latest revision as of 21:12, 21 October 2021

pDawn-B0030-blrA-rrnB T1

pDawn promoter system can be activated by blue light. blrA can be activated by blue light and produce reactive oxygen species(ROS).

Biology and Usage

pDawn is a kind of regulation system that has been illustrated effectively in Vibrio natriegens which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site In its downstream.

BlrA, originating from Bacillus subtilis, is a blue light-induced GTP-binding receptor, which possesses the LOV domain and autofluorescence. LOV domain enables it to be activated by blue light and produce ROS, which can damage the bacterial structure.

We take blrA as the core of our kill switch, combine it with inducible promotor system, terminator and a series of necessary elements. The whole part is inserted into a plasmid backbone and finally transformed into E.coli to gain enough correct and functional circuits. The circuits are transformed into Vibrio.natriegens. We choose growth curve and CFU to measure the killing effect of BlrA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2171
Illegal XhoI site found at 3851
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542
Illegal AgeI site found at 3703
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525
Illegal BsaI.rc site found at 3602
Illegal SapI site found at 3920

@@ Line 5: / Line 5: @@
 pDawn promoter system can be activated by blue light. blrA can be activated by blue light and produce reactive oxygen species(ROS).
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Biology and Usage===
+pDawn is a kind of regulation system that has been illustrated effectively in ''Vibrio natriegens'' which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site In its downstream.
+BlrA, originating from ''Bacillus subtilis'', is a blue light-induced GTP-binding receptor, which possesses the LOV domain and autofluorescence. LOV domain enables it to be activated by blue light and produce ROS, which can damage the bacterial structure.
+We take ''blrA'' as the core of our kill switch, combine it with inducible promotor system, terminator and a series of necessary elements. The whole part is inserted into a plasmid backbone and finally transformed into ''E.coli'' to gain enough correct and functional circuits. The circuits are transformed into ''Vibrio.natriegens''. We choose growth curve and CFU to measure the killing effect of BlrA.
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