Difference between revisions of "Part:BBa K3739062"

Latest revision as of 21:09, 21 October 2021

pDawn-B0030-GFP-rrnB T1

pDawn promoter system can be activated by blue light.

Biology and Usage

pDawn is a kind of regulation system that has been illustrated effectively in Vibrio natriegens which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site In its downstream.

Gfp codes for a green fluorescence protein. The expression level of GFP can be used to characterize the effect of blue light induction in pDawn system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2171
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 195
Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 582
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 1094
Illegal NgoMIV site found at 1184
Illegal AgeI site found at 414
Illegal AgeI site found at 1542
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1643
Illegal BsaI.rc site found at 525

@@ Line 5: / Line 5: @@
 pDawn promoter system can be activated by blue light.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Biology and Usage===
+pDawn is a kind of regulation system that has been illustrated effectively in ''Vibrio natriegens'' which based on optogenetics. Under the irradiation of blue light, a series of regulatory elements will work on regulating the expression of genes that inserted into multiple cloning site In its downstream.
+''Gfp'' codes for a green fluorescence protein. The expression level of GFP can be used to characterize the effect of blue light induction in pDawn system.
 <!-- -->