Difference between revisions of "Part:BBa K4006002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Design considerations include the codon optimization for the chloroplast of C. reinhardtii through the open source tool Chlamy Sequence Optimizer.
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This part was designed for Gibson assembly with the plasmid backbone pASapI. It includes SapI restriction sites on both ends to create a Golden Gate compatible plasmid for future use with other parts. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii.
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===Source===
 
===Source===

Revision as of 20:58, 21 October 2021


MT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 224
    Illegal SapI.rc site found at 31


Design Notes

This part was designed for Gibson assembly with the plasmid backbone pASapI. It includes SapI restriction sites on both ends to create a Golden Gate compatible plasmid for future use with other parts. It is codon optimized for use in the chloroplast of Chlamydomonas reinhardtii.

Source

BBa_K3275000

References