Difference between revisions of "Part:BBa K245114:Experience"
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− |   Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model. | + |    Through the modeling built with substituting parameters from published articles, the growth of <i>E. coli</i> and <i>P. gingivalis</i> under the effect of LL-37 would be inhibited significantly. However, the figure also shows that <i>E. coli</i> could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model. |
− | [[File:T--NCTU_Formosa--bacteria_V.S._time.png|500px|thumb|center|'''Figure 2. The growth curve of E. coli and P.gingivalis | + | [[File:T--NCTU_Formosa--bacteria_V.S._time.png|500px|thumb|center|'''Figure 2. The growth curve of <i>E. coli</i> and <i>P. gingivalis</i> |
]] | ]] | ||
− | [[File:T--NCTU_Formosa--bacteria_with_DenTeeth.png|500px|thumb|center|'''Figure 3. The growth curve of E. coli and P.gingivalis | + | [[File:T--NCTU_Formosa--bacteria_with_DenTeeth.png|500px|thumb|center|'''Figure 3. The growth curve of <i>E. coli</i> and <i>P. gingivalis</i> |
]] | ]] | ||
− |   Because P. gingivalis was in the RG2, so we could not do the LL-37 functional test by | + |    Because <i>P. gingivalis</i> was in the RG2, so we could not do the LL-37 functional test by inhibiting the growth of <i>P. gingivalis</i>. After reading some related papers, we found that the killing rate of <i>E. coli</i> was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test. |
[[File:T--NCTU_Formosa--bacteriatable.png|500px|thumb|center|'''Table 1. Parameters of the sterilization system of LL37 | [[File:T--NCTU_Formosa--bacteriatable.png|500px|thumb|center|'''Table 1. Parameters of the sterilization system of LL37 | ||
]] | ]] |
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