Difference between revisions of "Part:BBa K3720003"
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In fact, we introduced a plasmid incorporating this part into our E. coli K-12 strain. We then mixed 40㎕ of liquid culture medium of this E. coli with 1.0㎕ of D-luciferin (15 mg/ml) and subsequently injected CO and measured the luminescence. Although quantitative measurements could not be conducted because there is no proper device in our lab for observing bioluminescence. However, as shown in the picture, we can see with the naked eye that the color of the tube injected with carbon monoxide has changed. | In fact, we introduced a plasmid incorporating this part into our E. coli K-12 strain. We then mixed 40㎕ of liquid culture medium of this E. coli with 1.0㎕ of D-luciferin (15 mg/ml) and subsequently injected CO and measured the luminescence. Although quantitative measurements could not be conducted because there is no proper device in our lab for observing bioluminescence. However, as shown in the picture, we can see with the naked eye that the color of the tube injected with carbon monoxide has changed. | ||
| − | <p>[[File:T--Qdai--result.png|500px|thumb|center|Fig.1 Color changes in bacterial culture <br>Left: LB medium+luciferin Right: LB medium+luciferin+CO gas]] </p> | + | <p>[[File:T--Qdai--result.png|500px|thumb|center|Fig.1 Color changes in bacterial culture <br>Left: LB medium+luciferin Right: LB medium+luciferin+CO gas]] </p> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:50, 21 October 2021
Firefly luciferase with pCooM, RBS (weak), and double terminator
pCooM is CooA-dependent promoter originates from Rhodospirillum rubrum. Since CooA binds to carbon monoxide and activates pCooM, it is possible to create a light emitting mechanism that reacts with carbon monoxide when this part used in combination with CooA.
In fact, we introduced a plasmid incorporating this part into our E. coli K-12 strain. We then mixed 40㎕ of liquid culture medium of this E. coli with 1.0㎕ of D-luciferin (15 mg/ml) and subsequently injected CO and measured the luminescence. Although quantitative measurements could not be conducted because there is no proper device in our lab for observing bioluminescence. However, as shown in the picture, we can see with the naked eye that the color of the tube injected with carbon monoxide has changed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 900