Difference between revisions of "Part:BBa K4041009"

Revision as of 20:49, 21 October 2021

pET-Yafl-Ulp1

This is for the surface display of the protein Yafl-Ulp1 to cleave the SUMO-protein partner into SUMO and protein. Which in turn purifies the protein of choice without the use of tags. This method allows easy centrifugation to purify protein in a relatively pure output.




Figure 2, Illustration of Cleavage system (brown Yafl Ulp1, Red fusion protein)

Source: https://amb-express.springeropen.com/articles/10.1186/s13568-020-00999-4

This system does not work well with system that does cannot bear the toxicity, like the BL21 (DE3) -gold cells, which has a high mRNA stability.


Figure 2, showing the expression of Yafl, pET-LO-SUMO and pET-LO-SUMO-tachyplesin system.


From the picture, it is suggested to use lysis resistant strains and other strains with high toxicity tolerance for the IPTG induction of the peptide, preferably in high enrichment broth including terrific broth or magic media.

Sequence and Features