Difference between revisions of "Part:BBa K4059014:Design"

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===Source===
 
===Source===
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https://pubmed.ncbi.nlm.nih.gov/23886957/
  
 
===References===
 
===References===

Latest revision as of 20:42, 21 October 2021


PsiH from Psilocybe cubensis with N-terminal substitution (4), E. coli K12 codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 833
    Illegal SapI.rc site found at 455


Design Notes

One of the chimera N-terminals which gave the best expression level from the referenced study

Source

https://pubmed.ncbi.nlm.nih.gov/23886957/

References