Difference between revisions of "Part:BBa K3861005"

Revision as of 20:12, 21 October 2021

pKF01

Introduction

iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of thyA (BBa_K3861003) and lldR (BBa_K3861004). The expression of thyA and lldR is driven by the synthetic constitutive promoter P_syn-thyA (BBa_K3861002) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF. We used this backbone as part of the composite part / backbone BBa_K3861026.

Biology

Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 997
Illegal XhoI site found at 2804
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ==='''Introduction'''===
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-iGEM Humboldt_Berlin 2021 standard backbone based on pSB1C3. It contains the standard pSB1C3 ori, <a href="https://parts.igem.org/Part:BBa_K3861003">BBa_K3861003</a> and <a href="https://parts.igem.org/Part:BBa_K3861004">BBa_K3861004</a>. We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
+iGEM Humboldt_Berlin 2021 standard backbone derived from pSB1C3. It contains the standard the high-copy-number ColE1/pMB1/pUC origin of replication. The chloramphenicol resistance cassette of pSB1C3 was replaced with the coding sequences of <I>thyA</I> (<a href="https://parts.igem.org/Part:BBa_K3861003">BBa_K3861003</a>) and <I>lldR</I>  (<a href="https://parts.igem.org/Part:BBa_K3861004">BBa_K3861004</a>). The expression of <I>thyA</I> and <I>lldR</I> is driven by the synthetic constitutive promoter P_syn-thyA (<a href="https://parts.igem.org/Part:BBa_K3861002">BBa_K3861002</a>) (Stringer et al., 2013). pKF01 still contains the binding sites for the standard sequencing/amplification primers for BioBrick parts, VF2 and VF.
+We used this backbone as part of the composite part / backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861026">BBa_K3861026</a>.
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