Difference between revisions of "Part:BBa K3747015"

 
 
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Particulate methane monooxygenase m1 mimic.  
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Particulate methane monooxygenase m1 mimic. pMMO-m1 is described in Kim et al 2019 [1]. In this paper active pMMO is shown using a scaffold and additional reductant duroquinol. The authors express several pMMO constructs on a high copy number plasmid under control of a T7 promotor with a lac operator. In this paper the authors construct several pMMO mimics and they measure methanol production. They construct mimics pMMO-m1 to m4, which all contain pmmoB1 and –B2 substructures either linked in series or parallel to an apo form of human heavy-chain ferritin (huHF), which works as a scaffold. This to mimic the active site in native particulate MMO, but in a soluble form.
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<partinfo>BBa_K3747015 parameters</partinfo>
 
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[1] Kim, H.J., Huh, J., Kwon, Y.W. et al. Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nat Catal 2, 342–353 (2019). https://doi.org/10.1038/s41929-019-0255-1

Latest revision as of 20:05, 21 October 2021


pMMO-m1

Particulate methane monooxygenase m1 mimic. pMMO-m1 is described in Kim et al 2019 [1]. In this paper active pMMO is shown using a scaffold and additional reductant duroquinol. The authors express several pMMO constructs on a high copy number plasmid under control of a T7 promotor with a lac operator. In this paper the authors construct several pMMO mimics and they measure methanol production. They construct mimics pMMO-m1 to m4, which all contain pmmoB1 and –B2 substructures either linked in series or parallel to an apo form of human heavy-chain ferritin (huHF), which works as a scaffold. This to mimic the active site in native particulate MMO, but in a soluble form.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 377
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 1043
  • 1000
    COMPATIBLE WITH RFC[1000]


[1] Kim, H.J., Huh, J., Kwon, Y.W. et al. Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nat Catal 2, 342–353 (2019). https://doi.org/10.1038/s41929-019-0255-1