Difference between revisions of "Part:BBa K4041005"

 
Line 3: Line 3:
 
<partinfo>BBa_K4041005 short</partinfo>
 
<partinfo>BBa_K4041005 short</partinfo>
  
−
Template for T7 transcription of crRNA for Vibrio pathogenic gene marker. Need to be used with a EcoRI cut for the transcription to end, to generate a short crRNA instead of transcription doesn't end. Designed on 100% conserved region.
+
Template for T7 transcription of crRNA for Vibrio pathogenic gene marker. Need to be used with a XbaI overnight clean cut or PCR for the transcription to end at break point, to generate a short crRNA instead of transcription doesn't end. Designed on 100% conserved region.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:54, 21 October 2021


TDH cas12a crRNA transcription device (crRNA1)

Template for T7 transcription of crRNA for Vibrio pathogenic gene marker. Need to be used with a XbaI overnight clean cut or PCR for the transcription to end at break point, to generate a short crRNA instead of transcription doesn't end. Designed on 100% conserved region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]