Difference between revisions of "Part:BBa K3739016"
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===Biology=== | ===Biology=== | ||
Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.<br/> | Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.<br/> | ||
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===Usage=== | ===Usage=== | ||
By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression.<br/> | By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression.<br/> | ||
Revision as of 19:47, 21 October 2021
AKTA-his-Mfp5
It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.
Biology
Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.
Usage
By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter, and the constructed plasmid was transformed into BL21(DE3) to verify its expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]