Difference between revisions of "Part:BBa K245114:Experience"
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= Functional Test = | = Functional Test = | ||
  After finishing the design of DenTeeth, we wanted to know whether its inhibition ability can have a function, so we designed the following experiment. |   After finishing the design of DenTeeth, we wanted to know whether its inhibition ability can have a function, so we designed the following experiment. | ||
− | <br>  By taking the method of dish culture and sticking up the filter paper, we dropped the DenTeeth on the filter in the center of the E. coli, DH5α with | + | <br>  By taking the method of dish culture and sticking up the filter paper, we dropped the DenTeeth on the filter in the center of the E. coli, DH5α with pET32a, plate. After twelve hours, a circular area around the spot of the DenTeeth formed, in which the bacteria colonies did not grow. Zone of inhibition proved that the LL-37 produced by DenTeeth could successfully secrete out and inhibit the growth of E. coli, DH5α with pET32a. |
[[File:T--NCTU_Formosa--Zone of inhibition.png|500px|thumb|center|'''Figure 4. DenTeeth inhibition zone result in LB plates. ( The materials added in plate were in the following condition, and each total volume is 20μL. (A) 30% hydrogen peroxide, (B) E. coli (BL21) with pSB1K3, (C) DenTeeth, (D) E. coli (BL21) with pSB1K3 (Cell Lysate), (E) DenTeeth (Cell Lysate), (F) PBS, (G) E. coli (BL21) with pSB1K3 + PBS, (H) DenTeeth + PBS, (I) LB broth (Centrifuging E. coli (BL21) with pSB1K3 after liquid culturing ), (J) LB broth (Centrifuging E. coli (BL21) with pSB1K3 after liquid culturing ) ) | [[File:T--NCTU_Formosa--Zone of inhibition.png|500px|thumb|center|'''Figure 4. DenTeeth inhibition zone result in LB plates. ( The materials added in plate were in the following condition, and each total volume is 20μL. (A) 30% hydrogen peroxide, (B) E. coli (BL21) with pSB1K3, (C) DenTeeth, (D) E. coli (BL21) with pSB1K3 (Cell Lysate), (E) DenTeeth (Cell Lysate), (F) PBS, (G) E. coli (BL21) with pSB1K3 + PBS, (H) DenTeeth + PBS, (I) LB broth (Centrifuging E. coli (BL21) with pSB1K3 after liquid culturing ), (J) LB broth (Centrifuging E. coli (BL21) with pSB1K3 after liquid culturing ) ) | ||
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