Difference between revisions of "Part:BBa K3905011"
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This composite part synthesises an anti-miRNA that is vital to the use of our AND gate toehold switch (part BBa_K3905006). This anti-miRNA is an RNA strand containing bases complementary to both the 517-5p and 210-3p micro-RNAs. This means that when both RNAs are present a complex can form that can be used as a trigger for our AND toehold switch. It is preceded by a T7 promoter for functional RNAs (Part:BBa_K1614000) which is best suited for our coded region. | This composite part synthesises an anti-miRNA that is vital to the use of our AND gate toehold switch (part BBa_K3905006). This anti-miRNA is an RNA strand containing bases complementary to both the 517-5p and 210-3p micro-RNAs. This means that when both RNAs are present a complex can form that can be used as a trigger for our AND toehold switch. It is preceded by a T7 promoter for functional RNAs (Part:BBa_K1614000) which is best suited for our coded region. | ||
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| + | <html> | ||
| + | <figure> | ||
| + | <img width="600px" src="https://2021.igem.org/wiki/images/thumb/0/03/T--City_of_London_UK--ComplexFormation.png/800px-T--City_of_London_UK--ComplexFormation.png"> | ||
| + | </figure> | ||
| + | <figcaption> | ||
| + | <i>Diagram of Trigger Complex formation.</i> | ||
| + | </figcaption> | ||
| + | </html> | ||
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Latest revision as of 19:23, 21 October 2021
T7 / gen3 210-3p-517-5p AND-Gate Anti-miRNA / Terminator
This composite part synthesises an anti-miRNA that is vital to the use of our AND gate toehold switch (part BBa_K3905006). This anti-miRNA is an RNA strand containing bases complementary to both the 517-5p and 210-3p micro-RNAs. This means that when both RNAs are present a complex can form that can be used as a trigger for our AND toehold switch. It is preceded by a T7 promoter for functional RNAs (Part:BBa_K1614000) which is best suited for our coded region.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]