Difference between revisions of "Part:BBa K4092001"

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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Agave Tequilana (SST1 gene), has a MFE of -35.90000153 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
 
Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Agave Tequilana (SST1 gene), has a MFE of -35.90000153 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.
  
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===Usage and Biology===
 
  
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===Usage and Biology===
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In order to have a more graphical approach of the comparison of the structures, we obtained an image of the secondary structure of the toeholds using the NUPACK package.
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<html>
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<figure class="col-sm-* img-wrapper">
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              <img src="https://2021.igem.org/wiki/images/thumb/3/37/T--Tec-Monterrey--th-a-2.png/258px-T--Tec-Monterrey--th-a-2.png"
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                class="description-image" />
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              <figcaption style="text-align: left" class="mt-1">
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                <strong>Fig.</strong> Toehold secondary structure at 37°C.
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              </figcaption>
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            </figure>
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</html>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 19:02, 21 October 2021


Toehold for Agave tequilana 2

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C for the detection of Agave Tequilana (SST1 gene), has a MFE of -35.90000153 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.


Usage and Biology

In order to have a more graphical approach of the comparison of the structures, we obtained an image of the secondary structure of the toeholds using the NUPACK package.

Fig. Toehold secondary structure at 37°C.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]