Difference between revisions of "Part:BBa K4080009:Design"
 
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The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
 
The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
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Addition of 6xHis-tag after TEV protease
 
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All of the parts are from a previous iGEM team that registered in the parts registry
 
All of the parts are from a previous iGEM team that registered in the parts registry
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===References===
 

Latest revision as of 19:01, 21 October 2021


Sec system (PhoA), TEV protease with universal expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Besides the burden value of each part and basic criteria like illegal restriction sites and any other stuff that could burden the assembly, there is no specific consideration to deal with
  • The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
  • Addition of 6xHis-tag after TEV protease
  • Linkers placed between parts : GGG or GSG

Source

All of the parts are from a previous iGEM team that registered in the parts registry