Difference between revisions of "Part:BBa K4080012:Design"
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The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
 
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Addition of 6xHis-tag after TEV protease
 
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Revision as of 18:57, 21 October 2021


Sec system (PhoA), TEV protease with T7 expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The system works under the T7 expression system, each part must be compatible and build strong-good relations
  • The position of the start codon at the start of the signal peptide and the double stop codon at the end of eCPX
  • Addition of 6xHis-tag after TEV protease
  • Linkers placed between parts : GGG or GSG

Source

All of the parts are from the previous iGEM team that registered in the parts registry.

References