Difference between revisions of "Part:BBa K4046770"

Revision as of 18:46, 21 October 2021

CMV - BS #2 with spacer - BS #2 - Kozak - tdTomato - bghA

This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and tdTomato gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations” section

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1025
Illegal PstI site found at 1751
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1025
Illegal PstI site found at 1751
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 614
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1025
Illegal PstI site found at 1751
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1025
Illegal PstI site found at 1751
1000
COMPATIBLE WITH RFC[1000]

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 This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and <I>tdTomato</I> gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations” section
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 <!-- Add more about the biology of this part here
 ===Usage and Biology===