Difference between revisions of "Part:BBa K3733042"

Revision as of 18:31, 21 October 2021

PhoA-azurin with a His-tag

Azurin is a copper-containing redox protein secreted by Pseudomonas aeruginosa. Azurin preferentially enters a variety of cancer cells and induces apoptosis or cell cycle arrest, thereby playing an anti-cancer effect. A 6×His tag was added in order to purify the protein. PhoA, a signal peptide of Sec pathway in Escherichia coli, have the capability to guide the LL-37 excrete out of the cell.

To further demonstrate that Azurin generated from our engineered bacteria could combine with p53 successfully, we mixed Azurin(without 6x His tag) with p53(with 6x His tag) and purified them together by a Ni-NTA column. The SDS-PAGE gel of combination verification shows below in Figure 16. Two correct stripes can be observed in the lane of the gel, which illustrates these two proteins can combine with each other.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

Huang F, Qianhui S, Zhaojie Q, et al. Anticancer actions of azurin and its derived peptide p28[J]. The protein journal, 2020, 39(2): 182-189.

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 </p>
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+To further demonstrate that Azurin generated from our engineered bacteria could combine with p53 successfully, we mixed Azurin(without 6x His tag) with p53(with 6x His tag) and purified them together by a Ni-NTA column. The SDS-PAGE gel of combination verification shows below in <b>Figure 16</b>. Two correct stripes can be observed in the lane of the gel, which illustrates these two proteins can combine with each other.
+</p>
+===Sequence and Features===
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3733042 SequenceAndFeatures</partinfo>